Top 20 Interview Questions & Answers for Molecular Biologist Roles – 2025

Are you a molecular biologist looking for a new job opportunity? Prepare for your interview with these 20 up-to-date questions that will assess your knowledge, experience, and cultural fit. Whether you're applying for a position in research, industry, or academia, these questions cover all key areas of the job.

General Questions

• 1. What initially sparked your interest in molecular biology?
  Answer: I have always been fascinated by the building blocks of life and how they interact with each other. Molecular biology combines my passion for biology and chemistry, making it the perfect field for me.
• 2. What experience do you have in a laboratory setting?
  Answer: I have completed several laboratory courses during my undergraduate and graduate studies, where I gained hands-on experience in techniques such as PCR, cloning, and gel electrophoresis. I have also worked as a research assistant in a molecular biology lab for two years, where I gained further experience in experimental design, data analysis, and troubleshooting.
• 3. Have you ever encountered difficulties in a project and how did you overcome them?
  Answer: During my graduate studies, I encountered a technical issue that delayed my research project. To overcome it, I consulted with my colleagues and supervisor, researched alternative methods, and modified my experimental design. It was a team effort, but we were able to successfully complete the project.
• 4. How do you stay updated on the latest advancements in molecular biology?
  Answer: I regularly attend conferences, workshops, and webinars to learn about the latest techniques and research in the field. I also read scientific journals and follow reputable molecular biology blogs and social media pages.
• 5. Can you walk us through your research process?
  Answer: Firstly, I review relevant literature and identify a research question. Then, I design experiments and gather materials and reagents. After performing the experiments, I analyze the data and draw conclusions. Finally, I present my findings through publications or presentations.

Technical Skills & Experience

• 6. What is your experience with DNA and RNA extraction techniques?
  Answer: I have experience with various methods of DNA and RNA extraction, including phenol-chloroform extraction, spin-column kits, and magnetic bead purification. I am also familiar with optimizing these techniques for different sample types and downstream applications.
• 7. Can you explain the difference between PCR and qPCR?
  Answer: Both PCR (polymerase chain reaction) and qPCR (quantitative real-time PCR) amplify specific DNA sequences. However, qPCR also provides real-time data on the amount of DNA amplified, whereas PCR does not. This makes qPCR useful for quantifying gene expression or detecting genetic mutations.
• 8. Have you used CRISPR in your research?
  Answer: Yes, I have used CRISPR-Cas9 gene editing in my research project. I designed guide RNAs, performed transfections, and analyzed the resulting genomic changes. I also optimized the protocol for my specific cell type.
• 9. How do you ensure accuracy and reproducibility in your experimental results?
  Answer: I maintain detailed records of my experiments, including protocols, reagents, and conditions used. I also perform replicates and use appropriate controls to account for any variability. Additionally, I regularly verify my results through independent methods.
• 10. What experience do you have with analyzing and interpreting large datasets?
  Answer: I have experience with bioinformatics tools such as BLAST, ClustalW, and NCBI databases for sequence analysis and comparison. I have also used statistical software such as R and GraphPad Prism for data visualization and analysis.

Behavioral Questions

• 11. Can you tell us about a time when you had to work under a tight deadline?
  Answer: During my research project, we had a submission deadline for a conference presentation. I had to quickly troubleshoot an unexpected issue and complete the necessary experiments within a week. With the help of my team, I was able to complete the project on time and deliver a successful presentation.
• 12. How do you handle conflicts with colleagues?
  Answer: I believe in open and respectful communication to resolve conflicts. I try to understand the other person's perspective and find a compromise. If necessary, I involve a mediator or seek guidance from my supervisor.
• 13. Can you give an example of a project where you showed initiative and leadership?
  Answer: During my graduate studies, I initiated a collaborative project with a neighboring lab which involved optimizing a new technique for our mutual research interests. I took the lead on coordinating experiments, analyzing data, and sharing results. The project was successful, and we published a joint publication.
• 14. How do you handle unexpected results in your research?
  Answer: I first reevaluate my experimental design and procedures to identify potential errors. Then, I repeat the experiments and perform additional controls if necessary to confirm the results. If the unexpected results persist, I consult with my colleagues for their input.
• 15. Can you describe a successful collaboration with colleagues outside your field?
  Answer: In my previous job, I collaborated with chemists to develop a new technique for protein purification. We shared our expertise and knowledge to troubleshoot and optimize the technique. It was a great learning experience, and the final product was successfully published.

Situational Questions

• 16. Imagine your experiment results in unexpected findings, and your supervisor suggests changing the project direction. How would you handle this situation?
  Answer: I would first discuss with my supervisor the reasons for the unexpected results and assess the potential impact on the project. If the change in direction is necessary, I would adapt my approach and revise my experimental design to align with the new direction.
• 17. If a technician made a mistake in preparing a reagent, and it affected your experiments, how would you handle this?
  Answer: I would calmly address the issue with the technician and assess the impact on my experiments. If possible, I would repeat the experiments using the correctly prepared reagent. If the mistake significantly affected the results, I would consult with my supervisor for further guidance.
• 18. How do you prioritize and manage multiple experiments simultaneously?
  Answer: I first establish which experiments are time-sensitive and prioritize those accordingly. Then, I plan my daily and weekly schedule, setting achievable goals and deadlines. I also communicate with my team to ensure we are working efficiently and help each other if necessary.
• 19. What would you do if a reagent or instrument you needed for an experiment was not available?
  Answer: I would first check with my colleagues if they have the reagent or instrument available. If not, I would inform my supervisor and devise an alternative approach or consider delaying the experiment until the necessary materials are available.
• 20. How do you handle working with difficult or demanding team members?
  Answer: I believe in maintaining a professional and respectful attitude towards all team members. I would try to understand their perspective and work together to find a solution. If necessary, I would seek guidance from my supervisor or HR to address the issue.